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spots function  (Oxford Instruments)


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    Structured Review

    Oxford Instruments spots function
    Spots Function, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 44293 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/spot+function/Imaris/pm42151476-216-6-9
    Average 99 stars, based on 44293 article reviews
    spots function - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    other:

    Article Title: Tumor-infiltrating bacteria disrupt cancer epithelial cell interactions and induce cell-cycle arrest
    Article Snippet: The detached cellular structures (≤500 μm 3 ) were masked in a separate channel using the Lifeact-GFP signal and the spatial coordinates were determined using the Spot function of the Imaris software ( ).

    Article Title: An Innate Immune Receptor Toll-1 converts chronic light stress into glial-phagocytosis
    Article Snippet: These red-only puncta were manually annotated using the Spot function in Imaris software (Bitplane).

    Article Title: The Arp2/3 complex controls the development of homeostatic microglia
    Article Snippet: A three-dimensional image was generated in the Imaris Surpass view.

    Article Title: The Arp2/3 complex controls the development of homeostatic microglia.
    Article Snippet: A threedimensional image was generated in the Imaris Surpass view.

    Article Title: An Innate Immune Receptor Toll-1 converts chronic light stress into glial-phagocytosis
    Article Snippet: To quantify ROS levels in photoreceptor R7/8 axons, imaging data were analyzed using IMARIS software (Bitplane).

    Article Title: An Innate Immune Receptor Toll-1 converts chronic light stress into glial-phagocytosis
    Article Snippet: Manual quantification was performed using the Spot function in Imaris software (Bitplane).

    Article Title: A human neuron alzheimer's disease model reveals barriers to senolytic translatability.
    Article Snippet: ROI for Synapsin, Homer-1, and PSD95 were defined using the spot function in IMARIS.

    Article Title: Tumor-infiltrating bacteria disrupt cancer epithelial cell interactions and induce cell-cycle arrest.
    Article Snippet: In brief Galeano Niñ o et al. show that extracellular bacteria dominate colorectal and oral tumor microniches, remodeling the microenvironment with reduced cell density, scattered epithelium, and low proliferation.. Fusobacterium induces G0G1 quiescence and 5-FU resistance.. Spatial profiling, models, and patient cohorts link Fusobacterium burden to poor therapy response, highlighting microbial-tumor interactions as therapeutic targets.



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    Oxford Instruments imaris spot tool function
    (A) Representative images from FiloQuant analysis used to quantify filopodia density in healthy and SMA patient astrocyte monocultures at baseline and after stimulating actin remodeling using the ATP depletion and recovery assay (NaN3 +R). CMFDA dye was used to visualize the cells (grey signal) and filopodia quantified by FiloQuant <t>tool</t> are highlighted in magenta. Scale bar: 30µm. (B) <t>Imaris</t> surface and mask tool applied on to individual healthy and SMA patient astrocyte cells at baseline and after ATP depletion and recovery assay. Scale bar: 15µm. (C) Quantification of filopodia density from FiloQuant analysis across baseline and treatment conditions for healthy and SMA patient astrocyte samples. One-way ANOVA with Bonferroni multiple comparison statistical testing; ****p<0.0001. (D) Optic density readout from CDC42-GTP G-LISA assay measuring the activated form of CDC42 across baseline and treatment conditions for healthy and SMA patient astrocyte samples. One-way ANOVA with Bonferroni multiple comparison statistical testing, p-values not statistically significant. N=5 (biological replicates), n= 4 (technical replicates).
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    Image Search Results


    (A) Representative images from FiloQuant analysis used to quantify filopodia density in healthy and SMA patient astrocyte monocultures at baseline and after stimulating actin remodeling using the ATP depletion and recovery assay (NaN3 +R). CMFDA dye was used to visualize the cells (grey signal) and filopodia quantified by FiloQuant tool are highlighted in magenta. Scale bar: 30µm. (B) Imaris surface and mask tool applied on to individual healthy and SMA patient astrocyte cells at baseline and after ATP depletion and recovery assay. Scale bar: 15µm. (C) Quantification of filopodia density from FiloQuant analysis across baseline and treatment conditions for healthy and SMA patient astrocyte samples. One-way ANOVA with Bonferroni multiple comparison statistical testing; ****p<0.0001. (D) Optic density readout from CDC42-GTP G-LISA assay measuring the activated form of CDC42 across baseline and treatment conditions for healthy and SMA patient astrocyte samples. One-way ANOVA with Bonferroni multiple comparison statistical testing, p-values not statistically significant. N=5 (biological replicates), n= 4 (technical replicates).

    Journal: bioRxiv

    Article Title: Astrocyte targeted SMN1 gene therapy and forskolin application improves astrocyte filopodia actin defects and motor neuron synaptic dysfunction in human SMA disease pathology

    doi: 10.64898/2026.03.26.714618

    Figure Lengend Snippet: (A) Representative images from FiloQuant analysis used to quantify filopodia density in healthy and SMA patient astrocyte monocultures at baseline and after stimulating actin remodeling using the ATP depletion and recovery assay (NaN3 +R). CMFDA dye was used to visualize the cells (grey signal) and filopodia quantified by FiloQuant tool are highlighted in magenta. Scale bar: 30µm. (B) Imaris surface and mask tool applied on to individual healthy and SMA patient astrocyte cells at baseline and after ATP depletion and recovery assay. Scale bar: 15µm. (C) Quantification of filopodia density from FiloQuant analysis across baseline and treatment conditions for healthy and SMA patient astrocyte samples. One-way ANOVA with Bonferroni multiple comparison statistical testing; ****p<0.0001. (D) Optic density readout from CDC42-GTP G-LISA assay measuring the activated form of CDC42 across baseline and treatment conditions for healthy and SMA patient astrocyte samples. One-way ANOVA with Bonferroni multiple comparison statistical testing, p-values not statistically significant. N=5 (biological replicates), n= 4 (technical replicates).

    Article Snippet: Using the Imaris spot tool function, we quantified the CD44 and pERM puncta within astrocyte filopodia ( ) which revealed a statistically significant decrease in pERM , CD44 , and co-localized CD44/pERM ( ) expression within SMA patient-derived astrocytes cultures compared to healthy astrocytes at baseline and after actin remodeling stimulation.

    Techniques: Comparison